Pair Name	Gambogic acid, Cisplatin
Disease Info	[ICD-11: 2B51]	Osteosarcoma		Investigative
Biological Phenomena	Induction-->Mitochondria-mediated apoptosis
Gene Regulation	Up-regulation	Expression	CASP8	hsa841
	Up-regulation	Expression	CASP9	hsa842
In Vitro Model	MG-63	Osteosarcoma	Homo sapiens (Human)	CVCL_0426
	HOS	Osteosarcoma	Homo sapiens (Human)	CVCL_0312
	U2OS	Osteosarcoma	Homo sapiens (Human)	CVCL_0042
Result	GA could increase the chemotherapeutic effect of CDDP in human osteosarcoma treatment through inducing the cell cycle arrest and promoting cell apoptosis

Pair Name	Gambogic acid, Cisplatin
Disease Info	[ICD-11: 2C25]	Lung cancer		Investigative
Biological Phenomena	Induction-->Blockade of cell cycle in G0/G1 phase
Gene Regulation	Down-regulation	Expression	ABCC2	hsa1244
	Up-regulation	Expression	BAX	hsa581
	Up-regulation	Cleavage	CASP3	hsa836
	Down-regulation	Expression	LRP1	hsa4035
In Vitro Model	A-549	Lung adenocarcinoma	Homo sapiens (Human)	CVCL_0023
Result	Combination of GA with cisplatin enhances the antitumor effects on cisplatin-resistant lung cancer cells by downregulating MRP2 and LRP expression.

Pair Name	Gambogic acid, Cisplatin
Disease Info	[ICD-11: 2B51]	Osteosarcoma		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Expression	CASP3	hsa836
	Up-regulation	Cleavage	CASP8	hsa841
	Up-regulation	Expression	FAS	hsa355
	Up-regulation	Expression	MAPK14	hsa1432
In Vitro Model	MG-63	Osteosarcoma	Homo sapiens (Human)	CVCL_0426
	HOS	Osteosarcoma	Homo sapiens (Human)	CVCL_0312
In Vivo Model	0.2 mL of MG63 cell suspension (1.5×10⁷ cells/ml) was subcutaneously injected into the right flank of mice
Result	Our results demonstrate that GA may be a new potent therapeutic agent useful for targeting human OS cells.